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The RNAqueous?-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free? DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:
? Highly concentrated RNA—for increased sensitivity in downstream assays
? Isolation of high-quality RNA from micro-scale samples
? Reagents/protocol for RNA recovery from LCM samples
How it works
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free? system included in the kit. The RNAqueous?-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
产品介绍：
| Sample Type (General): | Cells, Tissue |
|---|---|
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Downstream Application: | Laser Capture Microdissection (LCM), Northern Blotting, Reverse Transcriptase PCR (RT-PCR) |
| Elution Volume - max: | 20 μl |
| Final Product: | Total RNA |
| Isolation Technology: | Silica Spin Column |
| Product Line: | Ambion?, RNAqueous? |
| Product Size: | 50 preps |
| Sample Type (Specific): | Cell Cultures, Laser Capture Microdissection (LCM) |
| Scale: | Micro |
